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Isolation And Characterization Of Bacteria From Selected Refuse Dump Sites In Nigeria

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CHAPTER ONE

1.0 INTRODUCTION

Waste generation play an important role in our environment. With the doubling of population and changing lifestyle pattern of the inhabitants the quantity of municipal waste generated is increasing in an alarming rate (Scarpino et al., 2010). Most of this waste is subjected to dumping in a specified disposal yard.

The greatest challenge to the environmentalists is the eco friendly management of this waste and application of microorganisms in this context has got an age over other available technologies. Organic waste is consumed by the bacteria used as nutrients by the bacteria, and is no longer present to produce odors, sludge, pollution or unsightly mess.

When bacteria consume waste, they convert the waste into safe by products and in due course of this conversion they actually produce several metabolites to break down the complex waste into simple compounds. Soil microorganisms are increasingly becoming an important source in the search for industrially important molecules (Scarpino et al.,2010).

Extent of microbial diversity in nature is still largely unknown, thus there might be many more useful products yet to be identified from soil microorganisms. In soil 80 to 99% of microorganisms remain unidentified whereas these biological communities are known to play a dominant role in maintaining a sustainable biosphere (Maleicka et al., 2007).

Hence there is an immense possibility to screen effective bacterial strains from waste dump sites with valuable applications. To cope up with the demand for new organisms with properties of production of unique enzymes/ molecules for industrial application and waste degradation there have been a constant effort in isolating novel bacteria from diverse environment.

Accordingly, the present study was aimed to investigate bacterial strains from waste dumps ( Brodie et al., 2007).

Refuse dumps refer to areas or land sites where material wastes from several sources and processes are deposited. Refuse dumps include both municipal solid wastes and in dustrial wastes including liquid effluents containing heavy metals (Olanrewaju, 2002). Refuse dumps provide a rich source of microorganisms most of which are pathogenic (Odeyemi et al., 2011).

This is usually as a result of the attraction of rodents and vector insects for which the dump serves as shelter and food source (Donderski et al., 2000). Although it is known that vector insects and rodents can transmit various pathogenic agents of diseases such as amoebic and bacillary dysentery, typhoid fever, salmonel- losis, cholera, plague and so on.

A good percentage of these infections are caused by bacteria which are suspended in air around these refuse dumps which may later settle and cause contamination. Activities involving the disposal of solid wastes even if properly controlled with proper precautionary measures adopted may have adverse impact on the envi- ronment especially air since most of the dumps are open.

Microorganisms present in the refuse use the refuse as a food source. Under the anaerobic conditions typical in most dumps, these microorganisms convert the organic material in the refuse to methane and carbon dioxide ( Wathes et al., Β 2000).

1.1 AIM AND OBJECTIVES

AIM

The aim this study is to isolate and characterize bacteria from selected refuse dump sites in Kaduna metropolis.

1.2 OBJECTIVES

The specific objective were to:

Collect sample from refuse dump sites in Tudun wada and Rigasa Kaduna

Isolate and characterize bacteria from the sample collected.

 

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